Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength

YANG Lina; ZENG Qiping; CAO Yixiang; CAI Xiaohui; CHEN Jinshan

doi:10.3969/j.issn.1006-0111.2018.04.010

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Objective To establish a double wavelength method for the simultaneous determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A in compound caulis polygoni multiflori mixture. Methods The HPLC separation was performed on an Agilent ZORBAX SB-C₁₈ column (250 mm×4.6 mm, 5 μm), gradient eluted with the mixture of acetonitrile and 0.1% phosphoric acid as mobile phase, the flow rate was 1 ml/min, the detection wavelength was 210 nm and 320 nm, and the column temperature was 25℃. Results There were good linear relationships in the determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A (r ≥ 0.9997), with the average recovery rate of 102.33%, 100.29%, 100.18%, 99.95%, 99.23%, 101.61%, 99.28%, and the RSD were less than 3%. Conclusion The method was accurate, stable and reproducible and could be used for quality control of compound caulis polygoni multiflori mixture.

Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength

The Pharmacy Department, the 175 th Hospital of PLA/the Affiliated Southeast Hospital of Xiamen University, Fujian Zhangzhou 363000, China

Received Date: 2017-11-24

Rev Recd Date: 2018-05-24

Key words:

compound caulis polygoni multiflori mixture /
HPLC /
stibene glucoside /
Lignin glycosides /
asperosaponin Ⅵ

Abstract: Objective To establish a double wavelength method for the simultaneous determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A in compound caulis polygoni multiflori mixture. Methods The HPLC separation was performed on an Agilent ZORBAX SB-C₁₈ column (250 mm×4.6 mm, 5 μm), gradient eluted with the mixture of acetonitrile and 0.1% phosphoric acid as mobile phase, the flow rate was 1 ml/min, the detection wavelength was 210 nm and 320 nm, and the column temperature was 25℃. Results There were good linear relationships in the determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A (r ≥ 0.9997), with the average recovery rate of 102.33%, 100.29%, 100.18%, 99.95%, 99.23%, 101.61%, 99.28%, and the RSD were less than 3%. Conclusion The method was accurate, stable and reproducible and could be used for quality control of compound caulis polygoni multiflori mixture.

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Reference (11)

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Simultaneous determination of seven active components in compound caulis polygoni multiflori mixture by HPLC of double wavelength

doi: 10.3969/j.issn.1006-0111.2018.04.010

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通讯作者: 陈斌, [email protected]

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