Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 36 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2018  >  36(1): 50-54

QIU Lijuan, MENG Xin, HOU Shengshu, YANG Genjin, ZHONG Weiqing. Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 50-54. doi: 10.3969/j.issn.1006-0111.2018.01.010
Citation: QIU Lijuan, MENG Xin, HOU Shengshu, YANG Genjin, ZHONG Weiqing. Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 50-54. doi: 10.3969/j.issn.1006-0111.2018.01.010
shu

Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities

doi: 10.3969/j.issn.1006-0111.2018.01.010
  • 1.

    School of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China

  • 2.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2016-12-08
  • Rev Recd Date: 2017-04-25
  • Objective To study the anti-oxidation properties of Zn2+-quercetin complexes. Methods The binding stoichiometric ratios and the binding constant of Zn2+ to quercetin were measured with UV-Vis spectroscopy. The binding sites on quercetin were determined via 1H NMR spectroscopy. The antioxidant activities of Zn2+-quercetin complexes were compared to quercetin by scavenging DPPH radical method. The binding ratio of Zn2+ to quercetin depends on neutralization of phenol groups of quercetin. Results The binding ratio of Zn2+ to quercetin is 1:2 when less than two OH groups were deprotonated. The binding ratio is 1:1 when more than three OH groups were deprotonated. The apparent binding constant is 2.42×106 M-1 for the 1:1 Zn2+-quercetin complex. The 3'-OH and 4'-OH of quercetin are involved in the Zn2+ binding. The scavenging DPPH radical activity of Zn2+-quercetin complex is 2.3 times of quercetin. Conclusion These results provide a new insight to expand applications of this traditional Chinese medicine.
  • [1] Miles SL, McFarland M, Niles RM. Molecular and physiological actions of quercetin:need for clinical trials to assess its benefits in human disease[J]. Nutr Rev, 2014, 72(11):720-734.
    [2] Rani N, Velan LPT, Vijaykumar S, et al. An insight into the potentially old-wonder molecule-quercetin:the perspectives in foresee[J]. Chin J Integr Med, 2015, 1:1-16.
    [3] Nabavi SF, Russo GL, Daglia M. Role of quercetin as an alternative for obesity treatment:you are what you eat![J]. Food Chem, 2015, 179:305-310.
    [4] Gormaz JG, Quintremil S, Rodrigo R. Cardiovascular disease:A target for the pharmacological effects of quercetin[J]. Curr Top Med Chem, 2015, 15(17):1735-1742.
    [5] Xiao ZP, Wang XD, Peng ZY, et al. Molecular docking, kinetics study, and structure-activity relationship analysis of quercetin and its analogous as Helicobacter pylori urease inhibitors[J]. J Agric Food Chem, 2012, 60(42):10572-10577.
    [6] 夏玉明, 朴惠善, 许妍姬, 等. 槲皮素过渡金属锌配合物的合成[J]. 延边大学学报(自然科学版), 1999, 25(1):37-39.
    [7] de Souza RF, De Giovani WF. Synthesis, spectral and electrochemical properties of Al(Ⅲ) and Zn(Ⅱ) complexes with flavonoids[J]. Spectrochim Acta A Mol Biomol Spectrosc, 2005, 61(9):1985-1990.
    [8] Tan J, Wang B, Zhu L. DNA binding, cytotoxicity, apoptotic inducing activity, and molecular modeling study of quercetin zinc(Ⅱ) complex[J]. Bioorg Med Chem, 2009, 17(2):614-620.
    [9] Primikyri A, Mazzone G, Lekka C, et al. Understanding zinc(Ⅱ) chelation with quercetin and luteolin:a combined NMR and theoretical study[J]. J Phys Chem B, 2015, 119(1):83-95.
    [10] 曾旭, 叶丹荣, 林德昌, 等. 含铋配合物与奈瑟氏菌铁结合蛋白的反应性质研究[J]. 无机化学学报, 2011, 27(11):2179-2184.
    [11] 王海燕, 曾秀, 张成平, 等. 槲皮素金属螯合物的研究与应用[J]. 食品科学, 2013, 34(13):361-364.
    [12] de Souza RF, De Giovani WF. Antioxidant properties of complexes of flavonoids with metal ions[J]. Redox Rep, 2004, 9(2):97-104.
  • 加载中
通讯作者: 陈斌, [email protected]
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(4730) PDF downloads(649) Cited by()

Related
Proportional views

Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities

doi: 10.3969/j.issn.1006-0111.2018.01.010
  • 1. School of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
  • 2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2016-12-08
  • Rev Recd Date: 2017-04-25

Abstract: Objective To study the anti-oxidation properties of Zn2+-quercetin complexes. Methods The binding stoichiometric ratios and the binding constant of Zn2+ to quercetin were measured with UV-Vis spectroscopy. The binding sites on quercetin were determined via 1H NMR spectroscopy. The antioxidant activities of Zn2+-quercetin complexes were compared to quercetin by scavenging DPPH radical method. The binding ratio of Zn2+ to quercetin depends on neutralization of phenol groups of quercetin. Results The binding ratio of Zn2+ to quercetin is 1:2 when less than two OH groups were deprotonated. The binding ratio is 1:1 when more than three OH groups were deprotonated. The apparent binding constant is 2.42×106 M-1 for the 1:1 Zn2+-quercetin complex. The 3'-OH and 4'-OH of quercetin are involved in the Zn2+ binding. The scavenging DPPH radical activity of Zn2+-quercetin complex is 2.3 times of quercetin. Conclusion These results provide a new insight to expand applications of this traditional Chinese medicine.

QIU Lijuan, MENG Xin, HOU Shengshu, YANG Genjin, ZHONG Weiqing. Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 50-54. doi: 10.3969/j.issn.1006-0111.2018.01.010
Citation: QIU Lijuan, MENG Xin, HOU Shengshu, YANG Genjin, ZHONG Weiqing. Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant activities[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(1): 50-54. doi: 10.3969/j.issn.1006-0111.2018.01.010
shu

    HTML

Reference (12)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: [email protected]

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return