Paclitaxel loaded nanoparticles with cholesterol succinyl bletilla striata polysaccharide as a carrier

CHENG Nian; ZHAO Wencui; ZHANG Qi; WANG Yanping; HAN Dan; XIAO Xuanang

doi:10.3969/j.issn.1006-0111.2017.01.012

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Objective To explore the feasibility of bletilla striata polysaccharide as a drug delivery material. Method With hydrophobic cholesterol succinyl bletilla striata polysaccharide (CHSB) as the carrier, the model drug paclitaxel (PTX) loaded nanoparticles were prepared via dialysis method. The morphology of nanoparticles was observed by Transmission Electron Microscope (TEM). The particle size, distribution and zeta potential were characterized by Dynamic Light Scattering instrument (DLS). The entrapment efficiency, drug loading and in vitro release were determined by high performance liquid chromatography (HPLC). Differential Scanning Calorimetry (DSC) was used to confirm the drug form in the drug loaded nanoparticles. The in vitro antitumor activity of nanoparticles was assayed by MTT. The uptake of nanoparticles by QGY-7703 liver cancer cells was observed by fluorescence labeling method. Results The prepared nanoparticles had spherical shape with uniform particle size distribution. The drug was loaded in the interior of the nanoparticles. Drug loading and encapsulation efficiency were affected by the CHSB in certain range. The cytotoxicity of drug loaded nanoparticles on liver cancer cells was stronger than the free drug. The fluorescence of Rhodamine B labeled drug nanoparticles were observed in the cells. Conclusion Cholesterol succinyl bletilla striata polysaccharide (CHSB) is highly feasible as an insoluble drug carrier. It can be used as a potential Nano carrier material.

Paclitaxel loaded nanoparticles with cholesterol succinyl bletilla striata polysaccharide as a carrier

1. School of Pharmacy, Yan Bian University, Yanji 133000, China

2. No. 208 Hospital of PLA, Changchun 130062, China

Received Date: 2016-05-31

Rev Recd Date: 2016-09-29

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Abstract: Objective To explore the feasibility of bletilla striata polysaccharide as a drug delivery material. Method With hydrophobic cholesterol succinyl bletilla striata polysaccharide (CHSB) as the carrier, the model drug paclitaxel (PTX) loaded nanoparticles were prepared via dialysis method. The morphology of nanoparticles was observed by Transmission Electron Microscope (TEM). The particle size, distribution and zeta potential were characterized by Dynamic Light Scattering instrument (DLS). The entrapment efficiency, drug loading and in vitro release were determined by high performance liquid chromatography (HPLC). Differential Scanning Calorimetry (DSC) was used to confirm the drug form in the drug loaded nanoparticles. The in vitro antitumor activity of nanoparticles was assayed by MTT. The uptake of nanoparticles by QGY-7703 liver cancer cells was observed by fluorescence labeling method. Results The prepared nanoparticles had spherical shape with uniform particle size distribution. The drug was loaded in the interior of the nanoparticles. Drug loading and encapsulation efficiency were affected by the CHSB in certain range. The cytotoxicity of drug loaded nanoparticles on liver cancer cells was stronger than the free drug. The fluorescence of Rhodamine B labeled drug nanoparticles were observed in the cells. Conclusion Cholesterol succinyl bletilla striata polysaccharide (CHSB) is highly feasible as an insoluble drug carrier. It can be used as a potential Nano carrier material.

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Reference (7)

[1]	孙达锋,史劲松,张卫明,等. 白及多糖胶研究进展[J].食品科学,2009,30(3):296.
[2]	毕亚静,王艳萍,刘福强,等. 胆甾醇琥珀酰基白及多糖的制备及其理化性质研究[J]. 药学实践杂志,2013,31(3):220-222.
[3]	赵雪竹,刘洋,王艳萍,等. 原料纯度对白及多糖疏水改性反应取代度的影响[J].药学实践杂志,2015,33(3):221-225.
[4]	杨文智. 疏水改性普鲁兰多糖及其自组装载药纳米粒的研究.北京:北京协和医学院,2009.
[5]	李磊. 胆固醇基-羧甲基可德兰衍生物自聚集纳米粒子的制备以及作为抗肿瘤药物载体的研究. 北京:中国协和医科大学,2010.
[6]	Gupta PN, Jain S, Nehate C,et al.Development and evaluation of paclitaxel loaded PLGA:poloxamer blend nanoparticles for cancer chemotherapy[J].Int J Biol Macromol,2014,69:393-399.
[7]	Thomas RG, Moon MJ, Lee S.et al. Paclitaxel loaded hyaluronic acid nanoparticles for targeted cancer therapy:In vitro and in vivo analysis[J].Int J Biol Macromol,2015,72:510-518.

[1]	孙达锋,史劲松,张卫明,等. 白及多糖胶研究进展[J].食品科学,2009,30(3):296.
[2]	毕亚静,王艳萍,刘福强,等. 胆甾醇琥珀酰基白及多糖的制备及其理化性质研究[J]. 药学实践杂志,2013,31(3):220-222.
[3]	赵雪竹,刘洋,王艳萍,等. 原料纯度对白及多糖疏水改性反应取代度的影响[J].药学实践杂志,2015,33(3):221-225.
[4]	杨文智. 疏水改性普鲁兰多糖及其自组装载药纳米粒的研究.北京:北京协和医学院,2009.
[5]	李磊. 胆固醇基-羧甲基可德兰衍生物自聚集纳米粒子的制备以及作为抗肿瘤药物载体的研究. 北京:中国协和医科大学,2010.
[6]	Gupta PN, Jain S, Nehate C,et al.Development and evaluation of paclitaxel loaded PLGA:poloxamer blend nanoparticles for cancer chemotherapy[J].Int J Biol Macromol,2014,69:393-399.
[7]	Thomas RG, Moon MJ, Lee S.et al. Paclitaxel loaded hyaluronic acid nanoparticles for targeted cancer therapy:In vitro and in vivo analysis[J].Int J Biol Macromol,2015,72:510-518.

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Paclitaxel loaded nanoparticles with cholesterol succinyl bletilla striata polysaccharide as a carrier

doi: 10.3969/j.issn.1006-0111.2017.01.012

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通讯作者: 陈斌, [email protected]

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