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Volume 32 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2014  >  32(6): 434-439

CHANG Yinlong, XIAO liang, ZHENG Jiemin, WANG Qianqian, ZHANG Liming. Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 434-439. doi: 10.3969/j.issn.1006-0111.2014.06.009
Citation: CHANG Yinlong, XIAO liang, ZHENG Jiemin, WANG Qianqian, ZHANG Liming. Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 434-439. doi: 10.3969/j.issn.1006-0111.2014.06.009
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Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata

doi: 10.3969/j.issn.1006-0111.2014.06.009

  • Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2013-11-25
  • Rev Recd Date: 2014-07-07
  • Objective To investigate the influencing factors of the protein stability and hemolytic activity of tentacle extract (TE) from the jellyfish Cyanea capillata. Methods Effects of various factors and treatments on the protein stability and hemolytic activity of TE were explored by protein detection, hemolytic assay and SDS-PAGE analysis. Results TE caused a significant and dose-dependent hemolytic effect, and the HU50 of TE against 0.5% erythrocyte suspensions from SD rats was 226 μg/ml. A 40℃ water bath for 1 hour could effectively remove the contaminating proteins in TE. TE retained hemolytic activity at 4℃ for 28 days but it was unstable when kept at 25℃ over 3 days. TE was active in the range from pH 6.0 to 11.0 and the optimum pH was 8.0. Various buffer solutions had significantly different effects on the stability and hemolytic activity of TE, and a good salting-out effect was observed on the hemolytic protein of TE while the concentration of ammonium sulfate solutions was greater than 26%. Conclusion A 40℃ water bath for 1 hour could effectively remove the contaminating proteins in TE and reduce its viscosity. The optimum conditions for maintaining stability and hemolytic activity of TE were 4℃ and pH 8.0. The salting-out effect from 26% and more ammonium sulfate solutions would be conducive to the enrichment of hemolytic protein.
  • [1] Tibballs J. Australian venomous jellyfish, envenomation syndromes, toxins and therapy[J]. Toxicon, 2006, 48(7): 830-859.
    [2] Kang C, Munawir A, Cha M, et al. Cytotoxicity and hemolytic activity of jellyfish Nemopilema nomurai(Scyphozoa: Rhizostomeae)venom[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2009, 150(1): 85-90.
    [3] Helmholz H, Ruhnau C, Schutt C, et al. Comparative study on the cell toxicity and enzymatic activity of two northern scyphozoan species Cyanea capillata (L) and Cyanea lamarckii (Peron & Leslieur)[J]. Toxicon, 2007, 50(1): 53-64.
    [4] Brinkman DL, Burnell JN. Biochemical and molecular characterisation of cubozoan protein toxins[J]. Toxicon, 2009, 54(8): 1162-1173.
    [5] Suput D. In vivo effects of cnidarian toxins and venoms. Toxicon, 2009, 54(8): 1190-1200.
    [6] Xiao L, He Q, Guo YF, et al. Cyanea capillata tentacle-only extract as a potential alternative of nematocyst venom: its cardiovascular toxicity and tolerance to isolation and purification procedures[J]. Toxicon, 2009, 53(1): 146-152.
    [7] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72:248-254.
    [8] Rocha J, Peixe L, Gomes NCM, et al. Cnidarians as a source of new marine bioactive compounds-an overview of the last decade and future steps for bioprospecting[J]. Marine Drugs, 2011, 9(10): 1860-1886.
    [9] Feng J, Yu H, Xing R, et al. Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii kishinouye[J]. Toxicol In Vitro, 2010, 24(6): 1750-1756.
    [10] Chung JJ, Ratnapala LA, Cooke IM, et al. Partial purification and characterization of a hemolysin (CAH1) from Hawaiian box jellyfish (Carybdea alata) venom[J]. Toxicon, 2001, 39(7): 981-990.
    [11] Gusmani L, Avian M, Galil B, et al. Biologically active polypeptides in the venom of the jellyfish Rhopilema nomadica[J]. Toxicon, 1997, 35(5): 637-648.
    [12] Nomura JT, Sato RL, Ahern RM, et al. A randomized paired comparison trial of cutaneous treatments for acute jellyfish(Carybdea alata) stings[J]. Am J Emerg Med, 2002, 20(7): 624-626.
    [13] Li R, Yu H, Xing R, et al. Isolation, identification and characterization of a novel antioxidant protein from the nematocyst of the jellyfish Stomolophus meleagris[J]. Int J Biol Macromol, 2012, 51(3): 274-278.
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Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata

doi: 10.3969/j.issn.1006-0111.2014.06.009
  • Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2013-11-25
  • Rev Recd Date: 2014-07-07

Abstract: Objective To investigate the influencing factors of the protein stability and hemolytic activity of tentacle extract (TE) from the jellyfish Cyanea capillata. Methods Effects of various factors and treatments on the protein stability and hemolytic activity of TE were explored by protein detection, hemolytic assay and SDS-PAGE analysis. Results TE caused a significant and dose-dependent hemolytic effect, and the HU50 of TE against 0.5% erythrocyte suspensions from SD rats was 226 μg/ml. A 40℃ water bath for 1 hour could effectively remove the contaminating proteins in TE. TE retained hemolytic activity at 4℃ for 28 days but it was unstable when kept at 25℃ over 3 days. TE was active in the range from pH 6.0 to 11.0 and the optimum pH was 8.0. Various buffer solutions had significantly different effects on the stability and hemolytic activity of TE, and a good salting-out effect was observed on the hemolytic protein of TE while the concentration of ammonium sulfate solutions was greater than 26%. Conclusion A 40℃ water bath for 1 hour could effectively remove the contaminating proteins in TE and reduce its viscosity. The optimum conditions for maintaining stability and hemolytic activity of TE were 4℃ and pH 8.0. The salting-out effect from 26% and more ammonium sulfate solutions would be conducive to the enrichment of hemolytic protein.

CHANG Yinlong, XIAO liang, ZHENG Jiemin, WANG Qianqian, ZHANG Liming. Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 434-439. doi: 10.3969/j.issn.1006-0111.2014.06.009
Citation: CHANG Yinlong, XIAO liang, ZHENG Jiemin, WANG Qianqian, ZHANG Liming. Protein stability and hemolytic activity of tentacle extract from the jellyfish Cyanea capillata[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 434-439. doi: 10.3969/j.issn.1006-0111.2014.06.009
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