基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

曾棋平; 蔡小辉; 吴坤林; 杨丽娜; 曹毅祥; 陈锦珊

doi:10.3969/j.issn.1006-0111.2019.05.008

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

曾棋平, 蔡小辉, 吴坤林, 杨丽娜, 曹毅祥, 陈锦珊

文章导航 > 药学实践与服务 > 2019 > 37(5): 427-432

曾棋平, 蔡小辉, 吴坤林, 杨丽娜, 曹毅祥, 陈锦珊. 基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究[J]. 药学实践与服务, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

引用本文:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

Citation:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

doi: 10.3969/j.issn.1006-0111.2019.05.008

中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建漳州, 363000

基金项目: 医院青年苗圃课题（16Y024）；福建中医药大学临床专项校管课题（XB2017080）

Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis

Department of Pharmacy, No. 909 Hospital of Joint Logistic Support Force/Southeast Hospital Affiliated to Xiamen University, Zhangzhou 363000, China

摘要: 目的探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱；腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型，用ELISA法测定大鼠海马体5-HT的含量，采用正交投影偏最小二乘法分析谱效关系。结果从指纹图谱中共确定21个共有峰，12个峰的变量投影重要性（VIP）均>1.0，复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关，通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础，可为制剂的质量控制提供实验依据。
- 复方首乌藤合剂 /
- 指纹图谱 /
- 抗失眠 /
- 谱效关系 /
- 正交投影偏最小二乘法
Abstract: Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture. Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship. Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture. Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.
- compound caulis polygoni multiflori mixture /
- fingerprint /
- anti-insomnia /
- spectrum-effect relationship /
- orthogonal partial least squares

[1]	王忠,陈寅萤,张盈颖,等.多组分多靶点中药药理作用机制研究中的问题和解决策略[J].中国实验方剂学杂志,2018,24(5):1-6.
[2]	LEE S.Systems biologyA pivotal research methodology for understanding the mechanisms of traditional medicine[J].J Pharmacopunct,2015,18(3):11-18.
[3]	张雨,李恒,李克宁,等.复方中药网络药理学的研究进展[J].中成药,2018,40(7):1584-1588.
[4]	刁嘉茵,徐灿,王淑美,等.中药指纹图谱与药效相关性研究进展[J].药学研究,2018,37(3):165-168.
[5]	蔡靓,张倩,杨丰庆,等.中药谱效学的应用进展[J].中草药,2017,48(23):5005-5011.
[6]	冯鑫,房德敏,周永梅.谱效关系分析在中药组方研究中的应用进展[J].中国中医基础医学杂志,2018,24(3):422-427.
[7]	曾棋平,杨丽娜,曹毅祥,等.层次分析联合Box-Behnken效应面法优选复方首乌藤合剂提取工艺[J].中南药学,2017,15(1):48-52.
[8]	曾棋平,张荣.高效液相色谱法同时测定复方首乌藤合剂中3种成分的含量[J].中南药学,2015,13(8):869-872.
[9]	杨丽娜,曾棋平,蔡小辉,等.复方首乌藤合剂高效液相指纹图谱研究[J].中南药学,2018,16(1):27-30.
[10]	池婕,林兵,刘志宏,等.基于最小二乘回归分析法的雷公藤多苷片免疫抑制作用谱效关系研究[J].中草药,2015,46(18):2755-2758.
[11]	黎理,曾祥燕,谢凤凤,等.竹节蓼药材的HPLC指纹图谱建立及聚类分析[J].中国药房,2018,29(12):1640-1643.
[12]	卿勇军,刘文,杜平,等.基于灰色关联度分析戊己丸对胃蛋白酶抑制作用的谱效关系[J].中草药,2017,48(16):3390-3395.
[13]	秦霞,郑华,阮俊翔,等.基于正交投影偏最小二乘法的当归四逆汤谱效关系研究[J].广西医科大学学报,2015,32(3):357-361.
[14]	肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326.
[15]	魏歆然,郑雪娜,裴芸,等.大鼠失眠模型造模中对氯苯丙氨酸混悬液改良法的探讨[J].湖南中医药大学学报,2018,38(9):986-989.
[16]	王金璐,郭伟英.新益心宁神片镇静催眠作用的谱效关系研究[J].中药材,2017,40(7):1633-1637.

[1]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[2]	张艺昕, 关欣怡, 王博宁, 闻俊, 洪战英. 二氢吡啶类钙离子拮抗药物手性分析及其立体选择性药动学研究进展 . 药学实践与服务, 2024, 42(8): 319-324. doi: 10.12206/j.issn.2097-2024.202308062
[3]	戴菲菲, 傅翔, 陈琼年, 俞苏纯. 上海某二级医院革兰阴性菌流行特征的回顾性分析 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202305005
[4]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[5]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[6]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063
[7]	张成中, 朱雪艳, 卜其涛, 王宏瑞, 黄宝康. 基于网络药理学与分子对接预测鸡骨草特征图谱研究 . 药学实践与服务, 2024, 42(8): 350-358. doi: 10.12206/j.issn.2097-2024.202303048
[8]	何静, 安晔, 张朝绅. 复方黑参滴丸与复方黑参丸药效学实验比较研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404009
[9]	郭灵怡, 刘艳超, 高路, 刘瑞瑶, 吕权真, 俞媛. 醋酸卡泊芬净单硬脂酸甘油酯纳米粒抗白色念珠菌感染的增效作用研究 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202310043
[10]	毛泽玲, 文波. 大黄的HPLC指纹图谱及禁用农药的残留研究 . 药学实践与服务, 2024, 42(7): 297-304, 314. doi: 10.12206/j.issn.2097-2024.202310057
[11]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012

点击查看大图

计量

文章访问数: 3340
HTML全文浏览量: 353
PDF下载量: 339
被引次数: 0

基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

doi: 10.3969/j.issn.1006-0111.2019.05.008

中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建漳州, 363000

基金项目: 医院青年苗圃课题（16Y024）；福建中医药大学临床专项校管课题（XB2017080）

收稿日期: 2019-03-15
修回日期: 2019-05-22

关键词:

抗失眠 /

摘要: 目的探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱；腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型，用ELISA法测定大鼠海马体5-HT的含量，采用正交投影偏最小二乘法分析谱效关系。结果从指纹图谱中共确定21个共有峰，12个峰的变量投影重要性（VIP）均>1.0，复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关，通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础，可为制剂的质量控制提供实验依据。

English Abstract

Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis

Department of Pharmacy, No. 909 Hospital of Joint Logistic Support Force/Southeast Hospital Affiliated to Xiamen University, Zhangzhou 363000, China

Received Date: 2019-03-15
Rev Recd Date: 2019-05-22

Keywords:

compound caulis polygoni multiflori mixture /
fingerprint /
anti-insomnia /
spectrum-effect relationship /
orthogonal partial least squares

Abstract: Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture. Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship. Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture. Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.

引用本文:

Citation:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008