基于网络药理学的肝力保胶囊保肝作用机制研究

陈俊; 陈青山; 孙森; 赵亮; 张海; 张国庆

doi:10.3969/j.issn.1006-0111.2018.05.005

基于网络药理学的肝力保胶囊保肝作用机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.005

陈俊¹,
陈青山¹,
孙森¹,
赵亮¹,
张海²,
张国庆^1,

1.
第二军医大学附属东方肝胆外科医院药材科, 上海 200438
2.
同济大学附属第一妇婴保健院药材科, 上海 201204

Study on hepato-protective mechanism of Ganlibao capsule based on network pharmacology

1.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China
2.
Department of Pharmacy, Shanghai First Maternal and Infant Hospital Affiliated to Tongji University, Shanghai 201204, China

摘要: 目的对肝力保胶囊进行网络药理学研究，探讨肝力保胶囊保肝的作用机制。方法选取肝力保胶囊化学物质组成中的活性成分，采用反向药效团匹配的方法预测活性成分的作用靶点；在此基础上，采用Cytoscape软件和String数据库分别构建肝力保胶囊"活性成分-作用靶点"的分子调控网络及蛋白-蛋白相互作用网络，最后采用DAVID数据库对靶点进行基因功能和信号通路分析。结果筛选得到活性化合物19个，涉及作用靶点88个。网络分析结果表明，肝力保胶囊主要通过代谢过程、调节细胞死亡和对脂质的应答等生物过程，以及调节肿瘤坏死因子（TNF）、FoxO、Ras、ErbB、低氧诱导因子-1（HIF-1）和Toll样受体（TLR）信号通路来发挥保肝作用。结论肝力保胶囊保肝作用具有多成分、多靶点、多通路的特点，其可能通过调节细胞死亡、TNF、FoxO、Ras等相关通路发挥作用。
- 肝力保胶囊 /
- 保肝作用 /
- 网络药理学 /
- 分子机制
Abstract: Objective To study the mechanism of Ganlibao capsule on liver protection by network pharmacology. Methods The active ingredients of Ganlibao capsule were selected and the targets of these ingredients were predicted by the reverse pharmacophore method. The molecule-regulatory network and protein-protein interaction network of the component-action target were constructed by Cytoscape and the String database respectively. The gene function and signal pathway analysis of the targets were analyzed by DAVID database. Results Nineteen active compounds were screened from the Ganlibao capsule, which involved 88 targets. The results of the network analysis showed that Ganlibao capsule exerted hepato-protective effects mainly through biological processes such as metabolic processes, regulation of cell death and response to lipids, and regulation of TNF, FoxO, Ras, ErbB, HIF-1, and TLR signaling pathways. Conclusion Ganlibao capsule could protect liver by multi-component, multi-target and multi-pathway, which might be through the regulation of cell death, TNF, FoxO, Ras and other related pathways.
- Ganlibao capsule /
- hepato-protective effect /
- network pharmacology /
- molecular mechanism

[1]	王慧, 李洋, 周燕妮, 等. HPLC法同时测定肝力保胶囊中绿原酸及虎杖苷的含量[J]. 解放军药学学报,2015,31(4):338-340.
[2]	王慧, 路克军, 张国庆. 高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱的含量[J]. 第二军医大学学报,2012,33(11):1267-1269.
[3]	李洋, 王慧, 吕磊,等. HPLC-TOF/MS对肝力保胶囊化学成分的快速鉴别[J]. 中国实验方剂学杂志,2015:70-73.
[4]	KIBBLE M, SAARINEN N, TANG J, et al. Network pharmacology applications to map the unexplored target space and therapeutic potential of natural products[J]. Nat Prod Rep,2015,32(8):1249-1266.
[5]	ZHAO S, IYENGAR R. Systems pharmacology:network analysis to identify multiscale mechanisms of drug action[J]. Annu Rev Pharmacol Toxicol,2012,52:505-521.
[6]	LI S, ZHANG B. Traditional Chinese medicine network pharmacology:theory, methodology and application[J]. Chin J Nat Med,2013,11(2):110-120.
[7]	李刚, 许波, 梁学振,等. 基于网络药理学研究淫羊藿抗骨质疏松的分子机制[J]. 中国药理学通报,2018(2):267-273.
[8]	冯燕燕, 谢媛媛, 汪艳平, 等. 基于"药物-靶点-通路"网络的血必净注射液治疗脓毒症分子调控机制[J]. 药学学报,2017,52(4):556-562.
[9]	王珂欣, 高丽, 周玉枝, 等. 基于网络药理学的苦参碱抗肝癌作用及机制研究[J]. 药学学报,2017,52(6):888-896.
[10]	蒋园, 白云, 刘萍, 等. 黄芪甲苷对CCl₄所致小鼠肝损伤的影响[J]. 中药药理与临床,2015,31(6):24-27.
[11]	张雅雯, 邵东燕, 师俊玲, 等. 山奈酚生物功能研究进展[J]. 生命科学,2017,29(4):400-405.
[12]	陈剑明, 张声生, 吴震宇, 等. 虎杖苷对非酒精性脂肪肝保护作用的实验研究[J]. 中华中医药学刊,2015,33(5):1188-1191+1294-1295.
[13]	战旗, 李悦悦, 王晓俊, 等. 人血白蛋白在原发性肝癌肝切除术后的应用分析[J]. 药学实践杂志,2015,33(5):463-466.
[14]	GARCIA-MARTINEZ R, CARACENI P, BERNARDI M, et al. Albumin:pathophysiologic basis of its role in the treatment of cirrhosis and its complications[J]. Hepatology,2013,58(5):1836-1846.
[15]	ZHAO G, HATTING M, NEVZOROVA YA,et al. Jnk1 in murine hepatic stellate cells is a crucial mediator of liver fibrogenesis[J]. Gut,2014,63(7):1159-1172.
[16]	POLIVKA J Jr,JANKU F. Molecular targets for cancer therapy in the PI3K/AKT/mTOR pathway[J]. Pharmacol Ther,2014,142(2):164-175.
[17]	BRENNER C, GALLUZZI L, KEPP O, et al. Decoding cell death signals in liver inflammation[J]. J Hepatol,2013,59(3):583-594.
[18]	PAN X, ZHANG Y, KIM HG, et al. FOXO transcription factors protect against the diet-induced fatty liver disease[J]. Sci Rep,2017,7:44597.
[19]	DOWNWARD J. Targeting RAS signalling pathways in cancer therapy[J]. Nat Rev Cancer,2003,3(1):11-22.
[20]	SEKI E, PARK E, FUJIMOTO J. Toll-like receptor signaling in liver regeneration, fibrosis and carcinogenesis[J]. Hepatol Res,2011,41(7):597-610.

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基于网络药理学的肝力保胶囊保肝作用机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.005

Study on hepato-protective mechanism of Ganlibao capsule based on network pharmacology

计量

基于网络药理学的肝力保胶囊保肝作用机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.005

1. 第二军医大学附属东方肝胆外科医院药材科, 上海 200438

2. 同济大学附属第一妇婴保健院药材科, 上海 201204

English Abstract

Study on hepato-protective mechanism of Ganlibao capsule based on network pharmacology

1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China

2. Department of Pharmacy, Shanghai First Maternal and Infant Hospital Affiliated to Tongji University, Shanghai 201204, China

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基于网络药理学的肝力保胶囊保肝作用机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.005

Study on hepato-protective mechanism of Ganlibao capsule based on network pharmacology

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基于网络药理学的肝力保胶囊保肝作用机制研究

doi: 10.3969/j.issn.1006-0111.2018.05.005

1. 第二军医大学附属东方肝胆外科医院药材科, 上海 200438 2. 同济大学附属第一妇婴保健院药材科, 上海 201204

English Abstract

Study on hepato-protective mechanism of Ganlibao capsule based on network pharmacology

1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China 2. Department of Pharmacy, Shanghai First Maternal and Infant Hospital Affiliated to Tongji University, Shanghai 201204, China

全文HTML

目录

1. 第二军医大学附属东方肝胆外科医院药材科, 上海 200438

2. 同济大学附属第一妇婴保健院药材科, 上海 201204

1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China

2. Department of Pharmacy, Shanghai First Maternal and Infant Hospital Affiliated to Tongji University, Shanghai 201204, China