摘要:
目的 探寻黄连水煎液对大鼠溃疡性结肠炎的治疗作用。 方法 大鼠以二甲肼(DMH) 40 mg/kg皮下注射+3%葡聚糖硫酸钠(DSS)水溶液饮用诱导形成大鼠溃疡性结肠炎模型,给予黄连水煎液灌胃治疗5周,观察黄连水煎液对大鼠溃疡性结肠炎的治疗作用。 结果 与模型组相比,黄连水煎液各剂量组能够不同程度改善溃疡性结肠炎引起的大鼠体重增长过慢(P<0.05,P<0.01)。实验第10周各组大鼠结肠均有畸变隐窝灶(ACF)形成, 黄连水煎液各剂量组形成ACF的平均数量明显低于模型组(P<0.01。病理检查结果显示,模型组大鼠可见结肠组织出现了比较严重的不典型增生,腺体排列紊乱,细胞层次明显增多且拥挤,黏液分泌显著减少,黏膜上皮脱落,糜烂重,溃疡多且大,黏膜严重充血、水肿;采用黄连水煎液各剂量组的大鼠上述现象则有所改善,但出现了不同程度的细胞拥挤,核增大和复层。透射电镜结果显示,模型组大鼠出现上皮细胞核染色质聚集,细胞表面微绒毛排列紊乱、断裂现象,黄连水煎液各剂量组的大鼠绒毛排列稀疏,未见断裂。 结论 黄连水煎液对大鼠溃疡性结肠炎有显著的治疗作用。
Abstract:
Objective To study the effect of coptis decoction on rats with ulcerative colitis. Methods Rat ulcerative colitis model was induced by 1,2-dimethylhydrazine(DMH)(40 mg/kg s.c.)+3% dextran sodium sulfate solution(DSS)(freely drinking). The drugs were given orally once a day for 7 day per week. After 5 weeks, the body weight, the number of colon ACFs and the number of colon cancer in rats, as well as the morphological changes of rat colon tissues were evaluated. Results Coptis decoction significantly improved the dyscrasia induced by DMH+DSS, the both of body weight and general condition were better than those of control group(P<0.05,P<0.01). Coptis decoction also significantly inhibited ACF in the rats treated by DMH+DSS for 10 weeks(P<0.01). The model group showed typical inflamed mucosa, a nearly complete subversion of the normal surface architecture. Compared with the model group, coptis decoction in each dosage group could improve rat atypical hyperplasia, mucosal hyperemia, edema and cells in the crowded. Transmission electron microscope showed that, in epithelial nuclei chromatin aggregation in the model group, the cell surface microvilli arranged disorder, fault phenomenon. Compared with model group, the coptis decoction had improved significantly. Conclusion Coptis decoction had significant therapeutic effect on rat ulcerative colitis.
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