The chemical constituents and antioxidant activity of Ixeris sonchifolia
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摘要: 目的 对菊科(Compositae)苦荬菜属 (Ixeris)植物抱茎苦荬菜正丁醇萃取层中的化学成分进行分离与鉴定。 方法 采用反复硅胶柱色谱﹑重结晶、Sephadex LH-20柱色谱﹑开放ODS柱色谱、HPLC等方法进行分离纯化以获得单体化合物。通过理化性质及波谱数据鉴定这些单体化合物的化学结构,利用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)法评价这些化合物清除自由基的活性。 结果 从抱茎苦荬菜正丁醇萃取层中分离得到5个化合物,分别鉴定为(+)-松脂素-4-O-β-D-葡萄糖苷( 1 )、glochidioboside ( 2 )、3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 )、芒柄花苷( 4 )、大豆素( 5 )、木犀草素( 6 )、芹菜素( 7 )。利用DPPH和ABTS法测试了这7个化合物清除自由基的活性。 结论 化合物 1 ~ 5 为首次从苦荬菜属植物中分离得到,化合物 1 、 2 、 4 ~ 7 显示了较强的自由基清除活性。Abstract: Objective To study the chemical constituents of Ixeris sonchifolia. Methods Monomeric compounds were isolated by chromatography on silica gel column, Sephadex LH-20, ODS chromatography column and HPLC.Thestructure of the compound wasconfirmed on the basis of physio-chemical constants and spectroscopic analysis. 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 3-ethylbenzthiazoline-6-sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds. Results Five compounds were isolated from 70% ethanol-water extraction and their structures were identified as (+)-pinoresinol-4-O-β-D-glucopyranoside( 1 ), glochidioboside( 2 ), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 ), ononin( 4 ),daidzein( 5 ), luteolin ( 6 ), and apigenin ( 7 ). Meanwhile, antioxidant activities of all the isolates were evaluated by DPPHand ABTS. Conclusion Compounds 1-5 were isolated from this genus for the first time. The active results showed that 1, 2, 4-7 exhibited potent antioxidant activity.
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