基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究

杨宇; 王慧; 曹颖瑛; 朱臻宇

doi:10.3969/j.issn.1006-0111.2015.03.005

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基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究

杨宇, 王慧, 曹颖瑛, 朱臻宇

文章导航 > 药学实践与服务 > 2015 > 33(3): 209-212

杨宇, 王慧, 曹颖瑛, 朱臻宇. 基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究[J]. 药学实践与服务, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005

引用本文:

YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005

Citation:

YANG Yua, WANG Hui, CAO Yingying, ZHU Zhenyu. Metabolic analysis of miconazole-treated Candida albicans by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 209-212. doi: 10.3969/j.issn.1006-0111.2015.03.005

基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究

doi: 10.3969/j.issn.1006-0111.2015.03.005

1.
第二军医大学药学院药物分析学教研室, 上海 200433
2.
第二军医大学药学院新药研究中心, 上海 200433

Metabolic analysis of miconazole-treated Candida albicans by GC-MS

1.
School of Pharmacy, Second Military Medical University, Department of Drug Analysis, Shanghai 200433 China
2.
School of Pharmacy, Second Military Medical University, New Drug Research and Develepment Center, Shanghai 200433 China

摘要: 目的基于代谢组学方法研究咪康唑作用前后白念珠菌的代谢谱差异, 寻找潜在的生物标志物, 探讨药物作用机制。方法采用气相色谱-质谱方法测定咪康唑给药前后白念珠菌的代谢特征谱, 利用多元统计分析方法进行比较, 筛选潜在的生物标志物, 并探讨代谢物产生差异的原因。结果经分析, 筛选出23个潜在的生物标志物, 主要参与了氨基酸代谢、三羧酸循环、氧化应激、糖酵解和磷脂代谢等代谢路径。结论咪康唑通过影响多种代谢路径而发挥抗真菌活性。
- 代谢组学 /
- 气相色谱-质谱 /
- 白念珠菌 /
- 咪康唑
Abstract: Objective To investigate the metabolism in miconazole-treated Candida albicans, search for possible biomarkers and discuss the mechanism of miconazole. Methods GC-MS was employed to determine the metabolic fingerprint of Candida albicans before and after treatment with miconazole, and the difference based on multivariate was compared by statistical analysis, the potential biomarkers were screened out and the mechanism of miconazole was discussed. Results Twenty three metabolites was screened out as potential biomarkers, and they were primarily involved in amino acid metabolism, citrate cycle, glycolysis and lipid metabolism. Conclusion The antifungal activity of miconazole was played by affecting a variety of metabolic pathways.
- metabolomics /
- gas chromatography-mass spectrometry /
- Candida albicans /
- miconazole

[1]	Kriengkauykiat J, Ito JI, Dadwal SS. Epidemiology and treatment approaches in management of invasive fungal infections[J]. Clin Epidemiol, 2011, 3: 175-191.
[2]	Vandenbosch D, De Canck E, Dhondt I, et al. Genomewide screening for genes involved in biofilm formation and miconazole susceptibility in Saccharomyces cerevisiae[J]. FEMS Yeast Res, 2013, 13(8): 720-730.
[3]	Zhu CX, Yan L, Wang XJ, et al. Transposition of the Zorro2 retrotransposon is activated by miconazole in Candida albicans[J]. Biol Pharm Bull, 2014, 37(1): 37-43.
[4]	吴海棠,李祥,王添琦,等.黄芩素作用白假丝酵母菌的GC-MS代谢组学研究[J]. 第二军医大学学报, 2013, 34 (2): 184-189.
[5]	Calahorra M, Lozano C, Sanchez NS, et al. Ketoconazole and miconazole alter potassium homeostasis in Saccharomyces cerevisiae[J]. Biochim Biophys Acta, 2011, 1808 (1): 433-445.
[6]	Sanchez-Fresneda R, Guirao-Abad JP, Arguelles A, et al. Specific stress-induced storage of trehalose, glycerol and D-arabitol in response to oxidative and osmotic stress in Candida albicans[J]. Biochem Biophys Res Commun, 2013, 430 (4): 1334-1339.
[7]	Feehily C, O'Byrne CP, Karatzas KA. Functional γ-Aminobutyrate Shunt in Listeria monocytogenes: role in acid tolerance and succinate biosynthesis[J]. Appl Environ Microbiol, 2013,79 (1): 74-80.
[8]	Cao J, Barbosa JM, Singh NK, et al. GABA shunt mediates thermotolerance in Saccharomyces cerevisiae by reducing reactive oxygen production[J]. Yeast, 2013, 30 (4): 129-144.
[9]	Kobayashi D, Kondo K, Uehara N, et al. Endogenous reactive oxygen species is an important mediator of miconazole antifungal effect[J]. Antimicrob Agents Chemother, 2002, 46 (10): 3113-3117.
[10]	Shim EH, Livi CB, Rakheja D, et al. L-2-hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer[J]. Cancer Discov, 2014, 4 (11): 1290-1298.
[11]	Rawal Y, Qiu H, Hinnebusch AG. Accumulation of a threonine biosynthetic intermediate attenuates general amino acid control by accelerating degradation of Gcn4 via Pho85 and Cdk8[J]. PLoS Genet, 2014, 10 (7): e1004534.
[12]	Pasrija R, Panwar SL, Prasad R. Multidrug transporters CaCdr1p and CaMdr1p of Candida albicans display different lipid specificities: both ergosterol and sphingolipids are essential for targeting of CaCdr1p to membrane rafts[J]. Antimicrob Agents Chemother, 2008, 52 (2): 694-704.

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基于气相色谱-质谱技术对用咪康唑处理的白念珠菌的代谢组学研究

doi: 10.3969/j.issn.1006-0111.2015.03.005

1. 第二军医大学药学院药物分析学教研室, 上海 200433

2. 第二军医大学药学院新药研究中心, 上海 200433

收稿日期: 2014-10-21
修回日期: 2015-02-28

关键词:

摘要: 目的基于代谢组学方法研究咪康唑作用前后白念珠菌的代谢谱差异, 寻找潜在的生物标志物, 探讨药物作用机制。方法采用气相色谱-质谱方法测定咪康唑给药前后白念珠菌的代谢特征谱, 利用多元统计分析方法进行比较, 筛选潜在的生物标志物, 并探讨代谢物产生差异的原因。结果经分析, 筛选出23个潜在的生物标志物, 主要参与了氨基酸代谢、三羧酸循环、氧化应激、糖酵解和磷脂代谢等代谢路径。结论咪康唑通过影响多种代谢路径而发挥抗真菌活性。