芎归汤主要化学成分的HPLC-TOF-MS分析

吕亚男; 谭光国

doi:10.3969/j.issn.1006-0111.2015.01.012

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芎归汤主要化学成分的HPLC-TOF-MS分析

吕亚男, 谭光国

文章导航 > 药学实践与服务 > 2015 > 33(1): 49-52,71

吕亚男, 谭光国. 芎归汤主要化学成分的HPLC-TOF-MS分析[J]. 药学实践与服务, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

引用本文:

吕亚男, 谭光国. 芎归汤主要化学成分的HPLC-TOF-MS分析[J]. 药学实践与服务, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

Citation:

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

芎归汤主要化学成分的HPLC-TOF-MS分析

doi: 10.3969/j.issn.1006-0111.2015.01.012

解放军22医院, 青海格尔木 816000

基金项目: 全军医学科技青年培育项目(13QNT197)

Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS

No.22 Hospital of PLA, Golmud 816000, China

摘要: 目的通过高效液相色谱-飞行时间质谱(HPLC-TOF-MS)联用技术,对中药复方芎归汤中的主要化学成分进行有效鉴定。方法色谱分离采用资生堂 CAPCELL PAK C₁₈反相柱(100 mm×3.0 mm,3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.4 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,通过飞行时间质谱鉴定了芎归汤中47种主要成分,并对其药材来源进行了归属。结论通过HPLC-TOF-MS联用技术,为鉴定芎归汤中的化学成分建立起了一种快速、高效的分析方法。
- 芎归汤 /
- 高效液相色谱-飞行时间质谱 /
- 化学成分
Abstract: Objective To analyze chemical constituents of Xionggui Decotion by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF-MS). Methods A Shelloseido column(100 mm×3.0 mm,3 μm)was used to separate.The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute.The flow rate was 0.4 ml/min.TOF-MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF-MS was established to identify the chemical constituents of Xionggui Decotion.
- Xionggui Decotion /
- HPLC-TOF-MS /
- chemical constituents

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芎归汤主要化学成分的HPLC-TOF-MS分析

doi: 10.3969/j.issn.1006-0111.2015.01.012

解放军22医院, 青海格尔木 816000

基金项目: 全军医学科技青年培育项目(13QNT197)

收稿日期: 2013-08-09
修回日期: 2014-01-11

关键词:

芎归汤 /

高效液相色谱-飞行时间质谱 /

化学成分

摘要: 目的通过高效液相色谱-飞行时间质谱(HPLC-TOF-MS)联用技术,对中药复方芎归汤中的主要化学成分进行有效鉴定。方法色谱分离采用资生堂 CAPCELL PAK C₁₈反相柱(100 mm×3.0 mm,3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.4 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,通过飞行时间质谱鉴定了芎归汤中47种主要成分,并对其药材来源进行了归属。结论通过HPLC-TOF-MS联用技术,为鉴定芎归汤中的化学成分建立起了一种快速、高效的分析方法。