Seasonal dynamics and tissue distribution of endophytic fungi in Cephalotaxus fortunei Hook. f.
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摘要: 目的 研究不同季节药用植物三尖杉(Cephalotaxus fortunei Hook. f.)不同组织部位内生真菌的种类组成、数量和分布规律的差异。 方法 采用组织分离法分离出春、夏、秋、冬4次采样的三尖杉叶片、枝条和树皮中的内生真菌,根据形态分型后进行分子鉴定。 结果 从4次采样的3个组织部位中共分离得到内生真菌1 851株。根据培养特征共划分为77个形态型,分子鉴定为70个分类单元。研究表明,季节和组织对内生真菌定殖率、分离率和种类组成均有明显的影响,但是季节的影响远大于组织的影响。 结论 三尖杉内生真菌具有丰富的生物多样性,结合内生真菌丰度和多样性指数综合分析,春季为获得较多种类的内生真菌的最佳取样时间。Abstract: Objective To investigated the species composition, number and distribution of endophytes in medicinal plants Cephalotaxus fortunei Hook. f. in different seasons and different tissues. Methods Endophytic fungi were isolated by tissue-culture method and identified by ITS analysis. Results 1851 strains of endophytic fungi were isolated from the leaves, twigs and barks of C. fortunei which were sampled in spring, summer, autumn and winter. These stains were divided into 77 morphotype according to the cultural characteristics and were identified as 70 taxons by ITS analysis. The results suggested that effects of season and tissue on the colonization rate, isolation rate and species composition of endophytes were apparent, and effects of season were stronger than that of tissue. Conclusion There are abundant and multiple endophytie fungi in Cephalotaxus fortunei. According to the comprehensive analysis combined with richness and biodiversity indexes, spring was the best sampling time for getting most species of endophytes.
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