摘要:
目的 建立高效液相色谱法对中药石见穿中的熊果酸及齐墩果酸进行含量测定。 方法 采用高效液相色谱法,色谱柱Agilent HC18 (4.6 mm×250 mm,5 μm),流动相A相为0.1%甲酸水溶液,B相为甲醇,A:B=14:86,流速为1.0 ml/min,柱温20℃,紫外检测波长205 nm,进样量50 μl,运行时间为35 min。 结果 熊果酸和齐墩果酸能够达到基线分离,二者分别在11.55~462.0、5.265~210.6 μg/ml,呈良好的线性关系,日内、日间精密度均小于5%(n=3),平均回收率分别为100.47%(RSD=1.95%,n=6)、101.88%(RSD=2.98%,n=6)。 结论 该法简便、快捷,结果准确、重复性好,可用于石见穿中熊果酸和齐墩果酸的质量控制。
Abstract:
Objective To establish a method for determination of ursolie acid and oleanolic acid contents in Salvia chinensis Benth. Methods HPLC-DAD was used;the column was Agilent HC18 (4.6 mm×250 mm,5μm);mobile phase was A:0.1%(v/v)formic acid solution,B:methanol,A:B=14:86. The flow rate was 1.0 ml/min;the temperature of column was 20℃;the detection wavelength was set at 205 nm;the injection volume was 50 μl; the running time was 35 min. Results Ursolic acid and oleanolic acid were separated in baseline, the linear range was 11.55~462.0 μg/ml for ursolic acid and 5.265~210.6 μg/ml for oleanolic acid. The result of intra-day and inter-day precisions were both within 5%(n=3), and the average recovery was 100.47%(RSD=1.95%, n=6)and 101.88%(RSD=2.98%, n=6). Conclusion The method was rapid,convenient,accurate,and suitable for quality control of ursolic and oleanolic acid in Salvia chinensis Benth.
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