Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC
-
摘要: 目的 建立测定葛花中鸢尾苷和鸢尾苷元含量的高效液相色谱方法。 方法 采用美国Agilent-C18柱(4.6 mm×250 mm,5 μm),以甲醇-乙腈-水(2∶1∶2)为流动相,流速为1.0 ml/min;检测波长为264 nm,柱温为室温。 结果 鸢尾苷在2.0 ~120.0 μg/ml呈线性关系,r=0.999 8;平均加样回收率为99.7%,RSD为1.9%;鸢尾苷元在0.8~80.0 μg/ml呈线性关系,r=0.99Abstract: Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C18 column(4.6 mm×250 mm,5 μm),using a mobile phase with methanol-acetonitrile-water(2∶1∶2). Flow rate was 1.0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 ~120.0 μg/ml,r=0.999 8.The average recovery was 99.7%,RSD was 1.9%;The linear range of tectorigenin was 0.8 ~80.0 μg/ml,r=0.998 6.The average recovery was 99.4%,RSD was 1.7%. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.
-
Key words:
- Flos puerariae lobata /
- tectoridin /
- tectorigenin /
- HPLC
-
[1] 田代华.实用中药辞典.下卷[M].北京.人民卫生出版社,2002:1338,1897. [2] 尹俊亭,仲 英,孙敬勇,等.葛花化学成分的研究[J].中草药,2006,37(3):350. [3] 张淑萍,贺 云,刘柏涛,等.RP-HPLC测定野葛花中3种异黄酮[J].分析实验室,2006,25(2):74. [4] 张国峰,吴 瑕,白 雪,等.HPLC法同时测定野葛花中4种异黄酮成分的含量[J].沈阳药科大学学报,2009,26(1):40. [5] 刘立辉,李卫民,高 英. HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖糖苷、鸢尾苷、鸢尾苷元的含量[J].中国中药杂志,2010,35(17):2308. [6] Kim CS,Shin SM,Ha HK,et al. Study of substance change in flowers of Pueraria thunbergiana B enth. during storage[J].Arch Pharm Res,2003,26(3):210. [7] Niiho Y, Nakajima Y, Yamazaki T, et al. Simultaneous analysis of isoflavones and saponins in Pueraria flowers using hplc coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside[J]. J Nat Med, 2010,64(3):313. -
点击查看大图
计量
- 文章访问数: 5989
- HTML全文浏览量: 369
- PDF下载量: 864
- 被引次数: 0
下载:
