Anti-inflammatory chemical constituents from the stem of Ilex cornuta Lindl.et Paxt
-
摘要: 目的 研究枸骨（Ilex cornuta Lindl.et Paxt）茎的抗炎活性成分。 方法 运用80%乙醇提取、大孔吸附树脂、正反相硅胶、Sephadex-LH20色谱柱等多种方法进行分离和纯化，根据理化性质和波谱数据进行结构鉴定。采用脂多糖（LPS）诱导的小鼠巨噬细胞RAW264.7的NO生成模型对部分化合物的抗炎活性进行测试。 结果 共分离得到10个化合物，经结构鉴定分别为α-香树脂醇（ 1 ）、齐墩果酸（ 2 ）、熊果酸（ 3 ）、羽扇豆醇（ 4 ）、menisdaurin（ 5 ）、lithospermoside（ 6 ）、槲皮素（ 7 ）、芦丁（ 8 ）、汉黄芩苷（ 9 ）、5，2'-二羟基-6，7，8，3'-四甲氧基黄酮（ 10 ）。测试了化合物 2~8 对LPS诱导的小鼠巨噬细胞RAW264.7的NO生成抑制活性。 结论 化合物 5、6、9、10 为首次从该植物中分离得到。化合物 3 和 7 能较明显地抑制LPS诱导的小鼠巨噬细胞RAW264.7的NO生成。Abstract: Objective To investigate the anti-inflammatory chemical constituents from the stem of Ilex cornuta Lindl.et Paxt. Methods The 80% EtOH extract of the stem of Ilex cornuta was purified by macro-porous adsorption resin chromatography,positive and negative silica gel chromatography,and Sephadex LH-20 chromatography. The structures of the purified compounds were determined by spectroscopic analysis and comparison with the reported data. Inhibitory effect of LPS induced mouse macrophage RAW264.7 on NO production was used to test the anti-inflammatory activity of compounds 2-8 . Results Ten compounds were isolated and their structures were determined as α-amyrin ( 1 ),oleanolic acid ( 2 ),ursolic acid ( 3 ),lupeol ( 4 ),menisdaurin ( 5 ),lithospermoside ( 6 ),quercetin ( 7 ),rutin ( 8 ),baicalin ( 9 ),5,2'-dihydroxy-6,7,8,3'-tetra-methoxy flavone ( 10 ). Conclusion Compounds 5, 6, 9 , and 10 were isolated from this plant for the first time. Compounds 3 and 7 inhibited the NO production on LPS induced mouse macrophage RAW264.7 cell.
-
[1] 中国科学院中国植物志编辑委员会.中国植物志:第45(2)卷[M].北京:科学出版社,1999:85. [2] 苏继海,马正民. 药用植物枸骨人工栽培技术[J]. 中国林副特产,2013,(3):78-79. [3] 左文健,梅文莉,曾艳波,等. 枸骨的化学成分和药理活性研究进展[J]. 安徽农业科学,2011,39(27):16560-16562. [4] SHERMAN MP,AEBERHARD EE,WONG VZ,et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages[J]. Biochem Biophys Res Commun,1993,191(3):1301-1308. [5] 席贞,唐敏,王文静,等.日香桂花的化学成分研究(Ⅱ)[J].华西药学杂志,2011,26(3):216-220. [6] 罗华锋,林朝展,赵钟祥,等.铁冬青茎皮五环三萜类化学成分的研究(Ⅰ)[J].中草药,2011,42(10):1945-1947. [7] 宋文雍,马云保,陈纪军,等.八角茴香根的化学成分研究[J].中草药,2009,40(8):1216-1219. [8] 李媛,宋宝安,杨松,等.中草药玄参化学成分的研究[J].天然产物研究与开发,2012,24(1):47-51. [9] SEIGLER DS.Cyanogenic glycosides and menisdaurin from Guazuma ulmifolia,Ostrya virginiana Tiquilia plicata,and Tiquilia canescens[J].Phytochemistry,2005,66(13):1567-1580. [10] HAN QB,JIANG B,MEI SX,et al. Constituents from the roots of Semiaquilegia adoxoides[J]. Fitoterapia,2001,72(1):86-88. [11] 邱声祥,张壮鑫,周俊. 雀瓢的黄酮醇成分[J].云南植物研究,1990,12(2):227-228. [12] 白杰,马秀琴,陈卓尔,等. 恰麻古化学成分的研究[J]. 华西药学杂志,2016,31(3):221-223. [13] 周锡钦,梁鸿,路新华,等. 中药黄芩主要黄酮类成分及其生物活性研究[J]. 北京大学学报(医学版),2009,41(5):578-584. [14] NISHIKAWA K,FURUKAWA H,FUJIOKA T,et al. Flavone production in transformed root cultures of Scutellaria baicalensis Georgi[J]. Phytochemistry,1999,52:885-890. -
点击查看大图
计量
- 文章访问数: 3526
- HTML全文浏览量: 334
- PDF下载量: 397
- 被引次数: 0
下载:
