摘要:
目的: 观察益心口服液预处理对急性心肌缺血大鼠的保护作用并初步探讨其作用机制。 方法: Wistar雄性大鼠,随机分为假手术组、模型组、地尔硫革组、复方丹参滴丸组、益心口服液大、中、小剂量组,通过冠状动脉左前降支结扎建立急性心肌缺血模型,观察结扎缺血过程中各组大鼠心电图J点位移变化、左心室功能的改变(LVSP、LVEDP、±dp/dtmax)、血清超氧化物歧化酶(SOD)和丙二醛(MDA)含量以及形态学的变化。 结果: 与模型组相比,益心口服液各剂量组均能对抗冠脉结扎引起的J点抬高及左心室功能的衰退(P<0.05),其中以大、中剂量组效果最佳;益心口服液大、中剂量组同时能提高心肌缺血大鼠血清SOD含量、降低MDA含量,减轻缺血心肌病理形态学的改变;益心口服液大、中剂量组对抗心肌缺血损伤的作用优于地尔硫(艹卓)处理组,但与复方丹参滴丸组无显著性差异(P>0.05)。 结论: 益心口服液预处理对冠脉结扎引起的急性心肌缺血具有一定的保护作用,其保护作用可能涉及抗氧化应激损伤机制。
Abstract:
Objective: To investigate the protective effect of Yixin oral liquid(YOL)preconditoning on acute myocardial ischemia in rats. Methods: The model of acute myocardial ischemia was established on rats by left coronary artery ligation,then the rats were divided into 7 groups randomly:sham operation group,Model group,Diltiazem hydrochloride group,Fufang Danshen Dripping Pills group(FD) and various concentration of YOL groups(high,medium and low doses).The change of J point of ECG,the left ventricular function (LVSP,LVEDP,±dp/dtmax),the superoxide dismutase(SOD)and malonaldehyde(MDA)contents in serum,and the pathologic structure of ischemic rats were observed. Results: The YOL preconditoning could obviously antagonize J point raise and deterioration of left ventricular function compared with the model group(P<0.05),in which the high and medium dose groups with YOL are better;and there were no significant difference between FD group and the high and medium-dose with YOL group.The high and medium dose group with YOL raised SOD level and decreased MDA level in serum,and also ameliorated myocardial pathologic structure changes in acute ischemia rat. Conclusion: YOL preconditioning contributes to protective effect on acute myocardial ischemia in rats.
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