高效液相色谱法测定延胡索中延胡索乙素的含量
Determination of dl-Tetrahydropamatine in Yanhusuo by HPLC
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摘要: 目的 建立高效液相色谱法测定中药材延胡索中延胡索乙素的含量。方法 以AgiLent zobax Extend C18(250mm×4.6mm,5μm)为色谱柱,甲醇-0.1%磷酸(三乙胺调pH值至6.0)(55:45)为流动相,流速1.0mL/min,检测波长:280nm。结果 延胡索乙素在115-1376ng范围内线性关系良好,平均回收率为99.2%,RSD=1.5%(n=6)。结论 该方法简便,重现性好,可作为中药材延胡索的质量控制方法。Abstract: OBJECTIVE An HPLC method was developed for determination of dl-Tetrahydropamatine in Yan-husuo. METHOD:The column was ODS Agilent zobax Extend C18(4.6×250mm,5μm),mobile phase was Meth-anol-0.1%. phosphoric acid(adjust pH 6.0 with Triethylamine)(55:45). Detection wavelength was set at 280nm. RESULT:Linearity range was 115-1376ng. The average recovery was 99.2%, RSD=1.5%(n=6). CONCLUSION The method is accurate and can be used for quality control of Yanhusuo.
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Key words:
- Yanhusuo /
- dl-Tetrahydropamatine /
- HPLC
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