RP－HPLC测定葛根芩连片中葛根素的含量
Determination of puerarin in Gegen qinlian pian by RP-HPLC
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摘要: 本文报道RP-HPLC测定葛根芩连片中葛根素含量。采用Shim-PackCLC-ODS(15cm×6mmID)分析柱;北分厂ODS保护柱;流动相:甲醇-水(23:77);流速:1.0ml/min;色谱柱温度:35℃;检测波长:250nm;进样体积10μl;外标法计算含量。理论板数按葛根素峰计算不低于2000;葛根素浓度在5μg/ml-80μg/ml范围与色谱峰面积呈线性关系;R=0.9999;回收率及RSD分别是97.6%,1.8%。Abstract: A quantitative method was developed for the determination of puerarin in Gegen Qing lian Pian by reversed phase HPLC.Chromatographic conditions included column ODS-C(10),column temperature,35℃;detector UV,250nm;mobile phase,methanol-water(23:77),flow rate 1 ml/min;injecting volume,10μl;external standad.the number of theoretical plates calculated by puerarin peak was no less than 2000.The standard curve was linear in the concentration range of 5μg/ml-80μg/ml,and correlation coefficient was 0.9999.The average rccovery and the relative standsrd deviation were 97.6% and 1.8%,respectively.
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Key words:
- RP-HPLC /
- Gegen Qinglian Pian /
- Puerarin
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